Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study
Nagendrababu Venkateshbabu, Sultan Omer Sheriff, Kannathasan Sreedharan, Patel Amir Shahreza, Chitra Ebenezer, Neelakantan Prasanna, Davamani Fabian,
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( Nagendrababu Venkateshbabu ) - International Medical University School of Dentistry Department of Clinical Dentistry
( Sultan Omer Sheriff ) - International Medical University School of Dentistry Department of Clinical Dentistry
( Kannathasan Sreedharan ) - International Medical University School of Medicine
( Patel Amir Shahreza ) - International Medical University School of Medicine
( Chitra Ebenezer ) - International Medical University School of Health Sciences Division of Applied Biomedical Science and Biotechnology
( Neelakantan Prasanna ) - University of Hong Kong Faculty of Dentistry Discipline of Endodontology
( Davamani Fabian ) - International Medical University School of Health Sciences Division of Applied Biomedical Science and Biotechnology
Abstract
Objectives: To determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro.
Materials and Methods: Root dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity of S. epidermidis to root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-Whitney U test (p = 0.05).
Results: The hydrophobicity and adherence of S. epidermidis to dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p < 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p < 0.05).
Conclusions: The adherence of S. epidermidis to dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.
키워드
Adherence; Chlorhexidine; Ethylenediaminetetraacetic acid; Hydrophobicity; Sodium hypochlorite; Staphylococcus epidermidis
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